Journal: Scientific Reports

Article Title: Robotic radiation shielding system reduces radiation-induced DNA damage in operators performing electrophysiological procedures

doi: 10.1038/s41598-025-03686-1

Figure Lengend Snippet: Flow cytometry analysis of the percentage of pATM and γH2AX positive lymphocytes. Whole blood samples from operators were lysed, fixed, permeabilized, and stained with anti-CD3, pATM and γH2AX antibodies. A . Representative pseudo-color plot for lymphocyte gating according to forward and side scatter profile and expression of CD3. (B) Representative flow cytometric dot plots for pATM expression in CD3 + cells obtained from the operator pre-procedure, immediately after procedure, 4 h post, and 24 h following EP procedure. (C) Representative flow cytometric dot plots for γH2AX expression in CD3 + cells obtained from the operator pre-procedure, immediately after the EP procedure, 4 h post, and 24 h following the procedure.

Article Snippet: The cells were stained for 30 min in the dark with 1:50 diluted fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD3 antibody (130-113-128, Miltenyi Biotec), 1:160 diluted (0.125 μg) phycoerythrin (PE)-conjugated anti-human ATM Phospho (Ser1981) antibody (651204, BioLegend), and 1:50 diluted allophycocyanin (APC)-conjugated anti-human H2AX pS139 (γH2AX) REAfinity antibody (130-123-256, Miltenyi Biotech).

Techniques: Flow Cytometry, Staining, Expressing

Journal: Scientific Reports

Article Title: Effects of different conditioning regimens on HLA-mismatched microtransplantation and changes in fine immune indices in acute myeloid leukaemia

doi: 10.1038/s41598-024-70332-7

Figure Lengend Snippet: Experimental reagents.

Article Snippet: FITC-labelled Mouse Anti-Human CD3 Antibody , BD Bioscience, USA , 662965.

Techniques:

Journal: Cell Reports Medicine

Article Title: Chimeric antigen receptor-induced antigen loss protects CD5.CART cells from fratricide without compromising on-target cytotoxicity

doi: 10.1016/j.xcrm.2024.101628

Figure Lengend Snippet: T cells resistant to CD5.CART cell elimination are enriched for CD8 + effector T cells (A) Percentage of residual targets following coculture of CD5.CART cells with resting T cells, CCRF-CEM, or Jurkat tumor cells for 48 h at an E:T ratio of 1:3. Resting T cells were CD3 + MACS isolated from fresh peripheral blood mononuclear cells. p values calculated using one-way ANOVA with Dunnett’s correction. n = 3 donors. Data are represented as mean ± SD. (B) Freshly isolated T cells were cocultured for 36 h with autologous CD19.CART cells or CD5.CART cells. Residual target cells were sort-purified and subjected to CITE-Seq. ssRNA-seq clustering by Seurat is shown. (C) Jaccard Similarity Index of Seurat UMAP of autologous T cells cocultured with either CD19.CART cells (left) or CD5.CART cells (center). Table denoting enriched and depleted clusters (right). (D) CD4, CD8, CD45RA, and CD62L surface expression of T cells (CITE-Seq) cultured with either CD19.CART cells or CD5.CART cells overlaid on Seurat UMAP. Gradient denotes log UMI counts of surface transcripts from CITE-seq. (E) CD5 protein surface expression (orange, top) and CD5 transcript levels (green, bottom) in residual T cells after coculture with either CD19.CART cells or CD5.CART cells overlaid on Seurat UMAP. Gradient denotes log UMI counts of surface and gene transcripts from CITE-Seq and RNA-seq, respectively. (F) Heatmap denoting fold change of genes in enriched clusters 3, 4, and 5, or depleted cluster 0 and 2 vs. all other cells; clustering by Seurat. (G) Differential abundance neighborhood groups. Color denotes significantly upregulated/downregulated genes with fold change differences above in CD5 CART treatment (red) or below compared to CD19 CART treatment (blue) with half log threshold using Milo. (H) Differentially expressed genes between CD19.CART treatment (left) and CD5.CART treatment (right) using Milo. Genes upregulated under CD5.CART treatment on the right.

Article Snippet: Mouse anti-human CD3 FiTC (clone SK7) , BD Biosciences , Cat#349201; RRID: AB_400405.

Techniques: Isolation, Purification, Expressing, Cell Culture, RNA Sequencing Assay

Journal: Cell Reports Medicine

Article Title: Chimeric antigen receptor-induced antigen loss protects CD5.CART cells from fratricide without compromising on-target cytotoxicity

doi: 10.1016/j.xcrm.2024.101628

Figure Lengend Snippet: Long-term resistance of normal T cells to CD5.CART cells is mediated by the loss of CD5 gene expression (A) Gating strategy (top). CD5 expression of T cells in patient #5 treated with CD5.CART cells that produced an abbreviated expansion (bottom). (B) CD3 + T cells in peripheral blood of patients with T cell lymphoma (TCL) or T cell acute lymphoblastic leukemia (T-ALL) treated with CD5.CART cells (NCT03081910). (C) CD5 expression of T cells in patient #7 (top), #8 (middle), and #9 (bottom) with durable CD5.CART cell persistence and achieved complete response. (D) CD5 mRNA expression of healthy T cells isolated from a lymph node biopsy at week 5 in patient #7 (left) and week 4 in patient #8 (right) normalized to respective donors’ T cells pre-lymphodepletion. (E) T cell phenotype of healthy T cells based on CCR7 and CD45RA expression of patient #7, #8, and #9. Naive (CCR7 + /CD45RA + ), central memory (CCR7 + /CD45RA − ), effector memory (CCR7 − /CD45RA − ), TEMRA (CCR7 − /CD45RA + ), and CD4/CD8 T cell distributions in healthy circulating T cells in patient #7, #8, and #9.

Article Snippet: Mouse anti-human CD3 FiTC (clone SK7) , BD Biosciences , Cat#349201; RRID: AB_400405.

Techniques: Expressing, Produced, Isolation

Journal: Cell Reports Medicine

Article Title: Chimeric antigen receptor-induced antigen loss protects CD5.CART cells from fratricide without compromising on-target cytotoxicity

doi: 10.1016/j.xcrm.2024.101628

Figure Lengend Snippet:

Article Snippet: Mouse anti-human CD3 FiTC (clone SK7) , BD Biosciences , Cat#349201; RRID: AB_400405.

Techniques: Enzyme-linked Immunosorbent Assay, Antibody Labeling, Western Blot, Sequencing, CRISPR, Software